I want to create a new DataFrame based upon the columns of another DataFrame, for which I would like to do the following: 1. **Data Retrieval:** Take partial strings from column elements from DataFrame1 2. **Renaming Column Elements:** Combine these strings and place into 2 new columns in DataFrame2 An example of the DataFrames can be seen below, where DataFrame1 is my actual data and the DataFrame2 is the type of data frame that I am interested in obtaining: import pandas as pd df1 = pd.DataFrame({'BGC Name': ['Hm106_120.region001', 'MBT13_26.region001', 'MBT23_64.region001', 'MBT36_100.region001'], 'GCF No': [1813, 1813, 1883, 1887], 'Organism': ['Streptomyces sp', 'Streptomyces sp', 'Listeria sp', 'Streptomyces sp'], 'BGC Class': ['PKSI', 'NRPS', 'PSKI', 'NRPS']}) df2 = pd.DataFrame({'GCF': ['PKSI_GCF1813', 'NRPS_GCF1813', 'PKSI_GCF1883', 'NRPS_GCF1887'],'Genome': ['Streptomyces_sp_Hm106', 'Streptomyces_sp_MBT13', 'Listeria_sp_MBT23', 'Streptomyces_sp_MBT36']}) print("DataFrame1 (Original DataFrame)") print(df1) print("DataFrame2 (Desired DataFrame)") print(df2) Snapshot can be found: [![SnapShot of The DataFrame[1]][1] The Code That I am trying to use #Dataframe Copies df1 = df1.copy() #self copy df1.columns = df1.columns.str.strip().str.lower().str.replace(' ', '_').str.replace('(', '').str.replace(')', '') df2 = df1.copy() #copy of original dataframe #Change Column Names df1.columns = ['BGC', 'GCF', 'Description', 'Product Prediction', 'BiG-SCAPE class', 'Organism'] df2.columns = ['BGC', 'GCF', 'Description', 'Product Prediction', 'BiG-SCAPE class', 'Organism'] #DataFrame Manipulation df2['GCF'] = df1['BiG-SCAPE class'].astype(str) + df2['GCF'].astype(str) df1[['BGC','BGC2']] = df1['BGC'].str.split('_',n=1,expand=True) df2['Genome'] = df1['Organism'].astype(str) + df1['BGC'].astype(str) df2.set_index('GCF') df2 = df2[['GCF','Genome']] df2['Genome'] = df2['Genome'].str.replace(' ', '_').str.replace('Unclassified','_').str.replace('__','_').str.replace('.','') print(df1) print("Updated table") print(df2) [1]: https://i.sstatic.net/uJEjn.png